Coding
Part:BBa_K5477021:Design
Designed by: Kate Malana Escobar Group: iGEM24_UCopenhagen (2024-09-26)
CYP3A4-MYC - Cytochrome P450 3A4 codon-optimized with MYC tag for Saccharomyces cerevisiae
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1483
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1540
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1483
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1483
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In the case of CYP3A4-MYC, the CYP3A4 enzyme retains its natural function as a cytochrome P450 enzyme, responsible for the oxidation and metabolism of a wide range of substrates, including pharmaceuticals, environmental toxins, and endogenous compounds. The addition of the MYC tag allows to easily detect and quantify CYP3A4 expression levels in cells. CYP3A4-MYC would provide the same broad detoxification functions as untagged CYP3A4, initiating the oxidative metabolism of drugs, pollutants, and toxins.
Source
Homo sapiens